Magnetic Resonance Using Low-Volume Samples
نویسندگان
چکیده
NMR spectroscopy allows measurements of very accurate values of equilibrium dissociation constants using chemical shift perturbation methods, provided that the concentrations of the binding partners are known with high precision and accuracy. The accuracy and precision of these experiments are improved if performed using individual capillary tubes, a method enabling full automation of the measurement. We provide here a protocol to set up and perform these experiments as well as a robust method to measure peptide concentrations using tryptophan as an internal standard.
منابع مشابه
Blood Flow Simulation in an Aorta with a mild coarctation Using Magnetic Resonance Angiography and Finite Volume Method
Coarctation of the aorta is one of the five main congenital cardiovascular failures, accounting for 6–8 percent of these failures. This research aimed to simulate the blood flow of a seventeen-year-old male teen with a mild coarctation at one-third of his aorta's descending branch. The simulation was performed by extracting the domain and the input pulsatile velocity signal as the boundary cond...
متن کاملSexual Dimorphism in Volume of the Cerebral Hemispheres and Lateral Ventricles in Schizophrenia Using Magnetic Resonance Imaging
Purpose: This study is designed to determine the sexual dimorphism pattern in volume of the cerebral hemispheres and lateral ventricles in schizophrenia using magnetic resonance imaging (MRI) and to compare it with normal sexual dimorphism pattern in healthy brains. Materials and Methods: This study is performed on 29 healthy volunteers (21 males, 8 females) and 29 patients suffered from schiz...
متن کاملBrain Structural Changes Caused by Autism Spectrum Disorder Based on Volumetric Analysis of Magnetic Resonance Images: A Review Study
Background and purpose: Autism spectrum disorder (ASD) is a psychiatric disorder which occurs in early years of life and causes various individual and social problems. Early detection of autism would help in taking necessary precautions and preventing its adverse side effects. Methods & Materials: In this paper, we reviewed the articles that have investigated brain structural changes caused by...
متن کاملThe Correlation between Left and Right Ventricular Ejection Fractions in Patients with Ischemic Heart Disease, Documented by Cardiac Magnetic Resonance Imaging
Introduction: The correlation between right and left ventricular ejection fractions (RVEF and LVEF, respectively) has been studied in only a small number of patients with a marked decrease in RVEF and LVEF. The aim of the present study was to compare LVEF and RVEF in patients with ischemic heart disease. RVEF and LVEF were measured by Cardiovascular Magnetic Resonance (CMR) imaging. Materials a...
متن کاملOptimization of clinical target volume delineation using magnetic resonance spectroscopic imaging (MRSI) in 3D conformal radiotherapy of prostate cancer
Background: For the purpose of individual clinical target volume assessment in radiotherapy of prostate cancer, MRSI was used as a molecular imaging modality with MRI and CT images. Materials and Methods: The images of 20 prostate cancer patients were used in this study. The MR and MRSI images were registered with CT ones using non-rigid registration technique. The CT based planning (BP), CT/MR...
متن کاملFabrication of New 3D Phantom for Measuring Geometric Distortion in Magnetic Resonance Imaging System
Introduction: Geometric distortion is a major shortcoming of magnetic resonance imaging (MRI), which has an important influence on the accuracy of volumetric measurements, an important parameter in neurology and oncology. Our goal is to design and construct a new three- dimensional phantom using a 3D printer in order to measure geometric distortion and its reproducibility in...
متن کامل